![]() The TCMSP, ChemMapper and STITCH databases were used to predict the protein targets of DBT. Anaemia disease-related protein target and compound of DBT databases were constructed. A metabolomics approach using Liquid Chromatography-Quadrupole-Time-of-Flight/Mass Spectrometry (LC/Q-TOFMS) was developed to perform the plasma metabolic profiling analysis and differential metaboliteswerescreened according to the multivariate statistical analysiscomparing the NC and BD groups, andthe hub metabolites were outliers with high scores of the centrality indices. Sprague-Dawley(SD) rats were randomly divided into 3 groups including control (NC, Saline), the DBT (at a dose of 8.10 g−1), and blood deficiency(BD) (Cyclophosphamide (APH)-andCyclophosphamide(CTX)-induced anaemia). Exploring new ways of treatment with DBT is useful. Mechanism of DBT action is wide ranging and unclear. DBT can promote iron ion binding and haemopoiesis activities, restrain inflammation, production of reactive oxygen, block apoptosis, and contribute significantly to the DBT treat anaemia.ĭanggui Buxue Tang (DBT) is a famous Chinese medicinal decoction. The results also showed that these targets were associated with iron ion binding, haemopoiesis, ROS production, apoptosis, inflammation and related signalling pathways. The gene-set enrichment analysis revealed that the targets are involved in iron ion binding, haemopoiesis, reactive oxygen species production, inflammation and apoptosis. A total of 288 targets were identified as major candidates for anaemia progression. 11 metabolic pathways are involved in the therapeutic effect of DBT in vivo S-adenosyl-l-methionine, glycine, l-cysteine, arachidonic acid (AA) and phosphatidylcholine(PC) were screened as hub metabolites in APH-and CTX-induced anaemia. The results shown DBT effectively treated anaemia in vivo. The gene enrichment analysis was used to analyse the relationship between proteins based on the relevant genetic similarity by ClueGO. Using the Cytoscape 3.2.1 to establish a phytochemical component-target protein interaction network and establish a component, protein and hub metabolite protein-protein interaction (PPI) network and merging the three PPI networks basing on BisoGenet. ![]() Danggui Buxue Tang (DBT) is a famous Chinese medicinal decoction.
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